Gene-edited cell line
Gene-edited cell lines inoreva maseru akagadziridzwa genetic kuburikidza nenzira inozivikanwa se gene editing.Kugadzirisa magene kunosanganisira kuita shanduko kuDNA yechipenyu, kazhinji nechinangwa chekudzidza majini chaiwo kana kunzwisisa mamwe maitiro ebhayoloji.
Uchishandisa gene editing tekinoroji, zvinokwanisika kuwana gene activation cell mitsara, kugogodza-out cell mitsara, point mutation, kana kugogodza-musero mitsara yemajini mune chaiwo maseru, ayo anogona kuzoshandiswa kudzidza geni basa, kusaina, nzira dzechirwere. , uye kukura kwezvinodhaka, pamwe nekuisa mazita emasero chaiwo.
Pane zvikonzero zvakati kuti nei gene-edited cell mitsara yakakosha patsvagiridzo yesainzi.Chekutanga, vanopa chishandiso chekudzidza kushanda kwemajini chaiwo.Nekushandura majini munzvimbo inodzorwa, masayendisiti anogona kuona mhedzisiro yekuchinja kwemajini pamaitiro esero kana mamwe maitiro ehupenyu.Ruzivo urwu runogona kubatsira mukunzwisisa zviri nani zvezvirwere zvemajini, pamwe nekuona zvinogona kurapa zvinangwa.
Pamusoro pezvo, gene-edited cell lines inogona kushandiswa kugadzira nekuyedza mishonga mitsva.Nekuunza shanduko chaiyo yemajini, masayendisiti anogona kugadzira mitsara yesero inotevedzera mamiriro echirwere, ichivagonesa kuongorora kushanda kwemishonga inogona.Izvi zvinobvumira kuti pave nemaitiro akanyatsojeka uye anoshanda ekugadzira zvinodhaka.
Uyezve, gene-edited cell mitsara ine mukana wekushandisa mukurapa kwekuvandudza.Nekugadzirisa majini ane chekuita nekuita kwesero kana kusiyanisa, masayendisiti anogona kusimudzira kugona kwekurapa kwe stem maseru kana kugadzira maseru anonyanya kukodzera kuisirwa.
Advantage
1. Gene-edited multi-locus panguva imwe chete;
2. kupa imwe-stop sevhisi kubva kusgRNA dhizaini uye synthesis kune cell line screening;
3. Ruzivo rwakapfuma mukugadzirwa kwemajini kubatsira mavheta ekugadziridza majini kubva pakugadzira chirongwa kusvika pakupera kwekuvaka.
Main Business
- Gene Knock-out Cell Lines
- Gene Activation Cell Lines
- Point mutant cell mitsara
- Knock-in Cell Lines
References
[1] Zhang S, Shen J, Li D, Cheng Y. Matanho mukuunza Cas9ribonucleoprotein yeCRISPR/Cas9 genome editing.Theranostics.2021 Jan1;11(2):614-648.doi: 10.7150/thno.47007.PMID: 33391496;PMCID: PMC7738854.