The Next-Generation Technology Expediting Mouse Model Creation
TurboMice™ Technology
Yakagadziridzwa naMingCeler kuburikidza neakateedzana optimizations ye tetraploid complementation.
NekubatanidzaPrecise Gene Editing tekinorojiuye optimized mouseEmbryonic Stem Cell Kugadzirira tekinoroji, isu tinokwanisa ikozvino kugadzirisa chero chinongedzo gene locus.
TurboMice™ Technology
Tekinoroji yedu yeTetraploid Complementation tekinoroji yatibvumira kuti tiwedzere zvakanyanya kuberekwa kwemakonzo kubva pa1% -5% kusvika pa30% -60% ine gene-edited stem cells, inenge ichienderana nekushanda kweyakajairika embryo kutamiswa.
MingCeler ndiyo kambani yekutanga munyika kubudirira kuita shanduko yeTetraploid Complementation tekinoroji kubva murabhoritari kuenda kumaindasitiri ekushandisa.
Traditional Technology
Tekinoroji yechinyakare yekuenzanisira mhuka, senge pronuclear microinjection uye ES yakanangana, inosanganisira kuberekesa zvizvarwa zviviri kusvika ku3 kuti vawane mbeva dzehomozygous genotype, dzinowanzotora mwedzi 6-8.
Iyi nzira yakareba inokanganisa zvakanyanya kufambira mberi uye kugona kwekuvandudzwa kwemishonga mitsva uye kutsvaga kwechirwere.
Quick Batch
1. kusvika 20 homozygous gene-edited mbeva mukati2-4 mwedzi.
2. ≈ 50 homozygous mice mukati mezvinotevera2 mwedzi.
3. 400+ homozygous mbeva mukatiMwedzi 8-12.
Nyaya Yenyaya muna 2020
TurboMice ™ tekinoroji yakasimudzirwa kuti ikurumidze kugadzira500 homozygoushumanized ACE2 mbeva mukati chete8 mwedziyeCOVID-19 yemishonga uye yekudzivirira zvidzidzo.
Flexible Strain Selection
Tsika dzechinyakare dzine zvipimo zvakakura mukusarudzika kwegonzo, nepo TurboMice ™ tekinoroji ichipa kuchinjika nemhando dzakasiyana siyana dzakazvarwa uye dzakabuda dzekusarudza kubva (kusanganisira Balb / c, ICR, C57BL/6, nezvimwewo).
● F0 mbeva dzakagadzirwa pachishandiswa tekinoroji yeTurboMice™ ndidzo dzinonangwa mbeva dzine sero rimwe chete.
● Nokudaro, iyo genetic material yeF0 mice yakafanana, kuderedza zvikanganiso zvekuedza zvinokonzerwa nekusiyana kwezvinhu zvakasiyana-siyana.
● Ergo, data yekuedza yekuongorora kushanda kwezvinodhaka uye zvimwe zviedzo zvinowirirana uye zvakavimbika.
Inochengetedza Yakanaka Genetic Kutendeseka
In Situ Precision Gene Kugadzirisa
● In situ precise gene editing ine nhanho dzemataurirwo emajini chaiwo uye matishu chaiwo.
● Mbeva dzeACE2 dzakagadzirwa nevanhu kuburikidza nehunyanzvi hwechinyakare dzinogadzirwa nekusumwa kweK18-ACE2 inosimudzira, iyo isingakwanise kunyatso gadzirisa nekuda kwekuisa zvisina tsarukano, zvichikonzera kushaikwa kwematishu ekutaura kweACE2 mune yakavakirwa munhu ACE2 mbeva modhi. .
● MingCeler's humanized ACE2 mbeva dzinoratidza kutaura chaiko munhengo dzakasiyana (giraidhi C uye D pamusoro), uye zvinobudirira kutevedzera hunhu hwekiriniki zvichitevera hutachiona hweSARS-CoV-2.
Yakasarudzika EnhancerPlus Platform
●MingCeler's proprietary EnhancerPlus chikuva inogona kubatsira vatengi vedu kuvandudza mazinga ekutaura kwemajini akaitwa nevanhu.
● Zvinokonzera makore ekuunganidza deta mu epigenetics uye cell fate determination tsvakurudzo, MingCeler's EnhancerPlus platform inogona kunyatsofanotaura genomic nzvimbo dzeEnhancers, zvichiita kuti dhizaini yekugadzira gene editing mazano-based.
● Nekuda kweizvozvo, majini anonangwa anogona kufanana zviri nani nematanho ekutaura uye mwero wemajini ekupedzisira.
Nyaya yenyaya: Humanization yeX gene.
● Leveraging the EnhancerPlus platform, iyo nzira yakagadziridzwa uye chiyero chekutaura chakaona kuwedzera kwemirairo mitatu yehukuru (ona pamusoro apa graphic), kusvika kune zvinodiwa pakugadzirwa kwezvinodhaka sezvinotsanangurwa nemutengi pazinga reprotein.
● Mushure mekupedza kugadzirwa kwepasi rose kweACE2 mbeva dzakagadzirwa nevanhu muna 2020, MingCeler akakwidziridza ACE2 humanized mbeva modhi kuburikidza ina iterations kuburikidza neEnhancerPlus optimization, nedanho rekutaura rehumunhu ACE2 rinozosvika pedyo neiyo endogenous kutaura kwembeva ACE2.
Mataurirwo mazinga eakaitwa munhu ACE2 munhengo dzakasiyana dzemakonzo emunhu
Multi-Locus Gene Kugadzirisa
● TurboMice™ tekinoroji ndiyo yakanakisa sarudzo pamusika yekugadzira akawanda-locus combinatorial gene-edited mbeva modhi, ichipa zvakanakira zvepamusoro-soro mukuisa nzvimbo, kuisirwa chidimbu chechidimbu, uye akawanda genetically modified loci, pasina allelic nyaya yekusarura.
● Tekinoroji yeTurboMice™ inobvumira kugadziridzwa kwemajini anosvika matatu panguva imwe chete kuti abudise homozygous multi-locus gene-edited mouse modhi kubva kune gene-edited embryonic stem cells, pasina kudiwa kwemaitiro anopedza nguva ekuberekesa/kuongorora, zvichipa zvakasiyana-siyana. yemhando yepamusoro-yakakosha yakaoma inodiwa patsvagurudzo yezvinodhaka.
● MingCeler yakabudirira kugadzira akasiyana-siyana akawanda-loci gene-edited mouse modhi munguva pfupi, zvichibva paACE2 humanized mbeva.
Yakareba Fragment Gene Kugadzirisa
TurboMice ™ tekinoroji inogonesa kurongeka kwemajini ezvimedu zveanopfuura 20kb, kufambisa kukurumidza kugadzirwa kwemhando dzakaomarara senge humanization, conditional knock-out (CKO), uye hombe fragment knock-in (KI).
Zvinyorwa
[1] Wang G, Yang ML, Duan ZL, Liu FL, Jin L, Long CB, Zhang M, Tang XP, Xu L, Li YC, Kamau PM, Yang L, Liu HQ, Xu JW, Chen JK, Zheng YT , Peng XZ, Lai R. Dalbavancin inosunga ACE2 kuvharidzira kudyidzana kwayo neSARS-CoV-2 spike protein uye inoshanda mukudzivisa hutachiona hweSARS-CoV-2 mumhando dzemhuka.Cell Res.2021 Jan;31(1):17-24.doi: 10.1038/s41422-020-00450-0.( IF: 20.507 )
[2] Liu FL, Wu K, Sun J, Duan Z, Quan X, Kuang J, Chu S, Pang W, Gao H, Xu L, Li YC, Zhang HL, Wang XH, Luo RH, Feng XL, Schöler HR , Chen X, Pei D, Wu G, Zheng YT, Chen J. Yekukurumidza chizvarwa cheACE2 humanized inbred mouse modhi yeCOVID-19 ine tetraploid complementation.Natl Sci Rev. 2020 Nov 24;8(2):nwaa285.doi: 10.1093/nsr/nwaa285.( IF: 16.693 )
Kuyerera Kwebasa
Zvese zvaunoda kuti uite ndezvekutipa yako gene shanduko inodiwa kune yako mbeva modhi.Zvichienderana nezvaunotaura, isu tichaisa pamwe yakagadziridzwa hurongwa hwekutanga, uye tichitevera imwe hurukuro uye kubvumirana, isu tichasaina chibvumirano chebasa rehunyanzvi.Mushure mekutanga kweprojekiti isu tinopa zvigadziriso nenguva nezve kufambira mberi.